300 series computing densitometer 4a Search Results


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anti cul4a  (Proteintech)
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Proteintech anti cul4a
E1, E2, and E3 interacting with the ACR, Ddb1, Crbn, Stub1, <t> Cul4a, </t> and Cul4b, are the most abundant UPS-linked proteins that bind to the ACR Table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); and the NSAF (4th to 8th columns. Phosphorylation of Thr 668 and/or Tyr 682 does not significantly alter binding of these five proteins.
Anti Cul4a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bodipy  (Echelon Biosciences)
93
Echelon Biosciences bodipy
E1, E2, and E3 interacting with the ACR, Ddb1, Crbn, Stub1, <t> Cul4a, </t> and Cul4b, are the most abundant UPS-linked proteins that bind to the ACR Table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); and the NSAF (4th to 8th columns. Phosphorylation of Thr 668 and/or Tyr 682 does not significantly alter binding of these five proteins.
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mb mme  (Malvern Panalytical)
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Malvern Panalytical mb mme
E1, E2, and E3 interacting with the ACR, Ddb1, Crbn, Stub1, <t> Cul4a, </t> and Cul4b, are the most abundant UPS-linked proteins that bind to the ACR Table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); and the NSAF (4th to 8th columns. Phosphorylation of Thr 668 and/or Tyr 682 does not significantly alter binding of these five proteins.
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value pipette  (Accumax Lab Devices)
93
Accumax Lab Devices value pipette
E1, E2, and E3 interacting with the ACR, Ddb1, Crbn, Stub1, <t> Cul4a, </t> and Cul4b, are the most abundant UPS-linked proteins that bind to the ACR Table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); and the NSAF (4th to 8th columns. Phosphorylation of Thr 668 and/or Tyr 682 does not significantly alter binding of these five proteins.
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24-hour holter electrocardiographic monitoring dms 300-4a  (DM Software Inc)
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DM Software Inc 24-hour holter electrocardiographic monitoring dms 300-4a
E1, E2, and E3 interacting with the ACR, Ddb1, Crbn, Stub1, <t> Cul4a, </t> and Cul4b, are the most abundant UPS-linked proteins that bind to the ACR Table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); and the NSAF (4th to 8th columns. Phosphorylation of Thr 668 and/or Tyr 682 does not significantly alter binding of these five proteins.
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agatoxin iva  (Alomone Labs)
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Alomone Labs agatoxin iva
E1, E2, and E3 interacting with the ACR, Ddb1, Crbn, Stub1, <t> Cul4a, </t> and Cul4b, are the most abundant UPS-linked proteins that bind to the ACR Table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); and the NSAF (4th to 8th columns. Phosphorylation of Thr 668 and/or Tyr 682 does not significantly alter binding of these five proteins.
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Image Search Results


E1, E2, and E3 interacting with the ACR, Ddb1, Crbn, Stub1, Cul4a, and Cul4b, are the most abundant UPS-linked proteins that bind to the ACR Table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); and the NSAF (4th to 8th columns. Phosphorylation of Thr 668 and/or Tyr 682 does not significantly alter binding of these five proteins.

Journal: The Journal of Biological Chemistry

Article Title: Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4 CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration *

doi: 10.1074/jbc.M116.733626

Figure Lengend Snippet: E1, E2, and E3 interacting with the ACR, Ddb1, Crbn, Stub1, Cul4a, and Cul4b, are the most abundant UPS-linked proteins that bind to the ACR Table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); and the NSAF (4th to 8th columns. Phosphorylation of Thr 668 and/or Tyr 682 does not significantly alter binding of these five proteins.

Article Snippet: The following antibodies were used in WB: anti-Ddb1 (catalog no. 6998s, lot 1, rabbit monoclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Pin-1 (catalog no. 3722s, lot 3, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Stub1 (catalog no. 2080s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Grb2 (catalog no. 3972s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Cul4a (catalog no. 14851-1-AP, lot 1, rabbit polyclonal antibody, ProteinTech, dilution 1:300); anti-Crbn (catalog no. HPA045910, lot Q103829, rabbit polyclonal antibody, Sigma, dilution 1:500); and anti-FLAG M2 (catalog no. A2220, lot SLBF8148, mouse monoclonal antibody, Sigma, dilution 1:1000).

Techniques: Binding Assay

Stub1 and CRL4CRBN E3 ligases bind distinct regions of the ACR. A, schematic diagrams of the structural domains of the ACR. Jcasp and Ccas are generated by a double γ-secretase and caspase cleavage of APP. The Thr668 and Tyr682 residues that are phosphorylated are underlined. B, Western blotting analysis of pulldowns shows that Crbn, Ddb1, Cul4a, specifically bind St-Ccas, St-CcasTyr(P), and St-CcasThr(P). Stub1 binds specifically St-JCasp. Two previously known APP interactors, Grb2 and Pin1, bind St-CcasTyr(P) and St-CcasThr(P), respectively. This evidence validates the proteomic approach used. In. indicates the input. The WB shown are representative of at least three independent experiments.

Journal: The Journal of Biological Chemistry

Article Title: Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4 CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration *

doi: 10.1074/jbc.M116.733626

Figure Lengend Snippet: Stub1 and CRL4CRBN E3 ligases bind distinct regions of the ACR. A, schematic diagrams of the structural domains of the ACR. Jcasp and Ccas are generated by a double γ-secretase and caspase cleavage of APP. The Thr668 and Tyr682 residues that are phosphorylated are underlined. B, Western blotting analysis of pulldowns shows that Crbn, Ddb1, Cul4a, specifically bind St-Ccas, St-CcasTyr(P), and St-CcasThr(P). Stub1 binds specifically St-JCasp. Two previously known APP interactors, Grb2 and Pin1, bind St-CcasTyr(P) and St-CcasThr(P), respectively. This evidence validates the proteomic approach used. In. indicates the input. The WB shown are representative of at least three independent experiments.

Article Snippet: The following antibodies were used in WB: anti-Ddb1 (catalog no. 6998s, lot 1, rabbit monoclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Pin-1 (catalog no. 3722s, lot 3, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Stub1 (catalog no. 2080s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Grb2 (catalog no. 3972s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Cul4a (catalog no. 14851-1-AP, lot 1, rabbit polyclonal antibody, ProteinTech, dilution 1:300); anti-Crbn (catalog no. HPA045910, lot Q103829, rabbit polyclonal antibody, Sigma, dilution 1:500); and anti-FLAG M2 (catalog no. A2220, lot SLBF8148, mouse monoclonal antibody, Sigma, dilution 1:1000).

Techniques: Generated, Western Blot

Ddb1, Crbn, Cul4a, and Cul4b bind to Ccas and Stub1 binds to JCasp Phosphorylation of Thr 668 and/or Tyr 682 does not significantly alter binding of Ddb1, Crbn, Cul4a, and Cul4b, whereas binding of Pin1 and Grb2 is dependent on phosphorylation of Thr 668 and Tyr 682 , respectively. Several of the other UPS-related proteins that are bound to the ACR bind to either Ccas or JCasp or both.

Journal: The Journal of Biological Chemistry

Article Title: Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4 CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration *

doi: 10.1074/jbc.M116.733626

Figure Lengend Snippet: Ddb1, Crbn, Cul4a, and Cul4b bind to Ccas and Stub1 binds to JCasp Phosphorylation of Thr 668 and/or Tyr 682 does not significantly alter binding of Ddb1, Crbn, Cul4a, and Cul4b, whereas binding of Pin1 and Grb2 is dependent on phosphorylation of Thr 668 and Tyr 682 , respectively. Several of the other UPS-related proteins that are bound to the ACR bind to either Ccas or JCasp or both.

Article Snippet: The following antibodies were used in WB: anti-Ddb1 (catalog no. 6998s, lot 1, rabbit monoclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Pin-1 (catalog no. 3722s, lot 3, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Stub1 (catalog no. 2080s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Grb2 (catalog no. 3972s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Cul4a (catalog no. 14851-1-AP, lot 1, rabbit polyclonal antibody, ProteinTech, dilution 1:300); anti-Crbn (catalog no. HPA045910, lot Q103829, rabbit polyclonal antibody, Sigma, dilution 1:500); and anti-FLAG M2 (catalog no. A2220, lot SLBF8148, mouse monoclonal antibody, Sigma, dilution 1:1000).

Techniques: Binding Assay

Binding of Ddb1, Cul4a, and Cul4b to ACR Thr(P)Tyr(P) requires Crbn Table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); NSAF of pulldown from WT mouse brains (4th column); NSAF of pulldown from Crbn -KO mouse brains (5th column). Binding of Stub1, Grb2, and Pin1 is independent of Crbn.

Journal: The Journal of Biological Chemistry

Article Title: Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4 CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration *

doi: 10.1074/jbc.M116.733626

Figure Lengend Snippet: Binding of Ddb1, Cul4a, and Cul4b to ACR Thr(P)Tyr(P) requires Crbn Table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); NSAF of pulldown from WT mouse brains (4th column); NSAF of pulldown from Crbn -KO mouse brains (5th column). Binding of Stub1, Grb2, and Pin1 is independent of Crbn.

Article Snippet: The following antibodies were used in WB: anti-Ddb1 (catalog no. 6998s, lot 1, rabbit monoclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Pin-1 (catalog no. 3722s, lot 3, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Stub1 (catalog no. 2080s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Grb2 (catalog no. 3972s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Cul4a (catalog no. 14851-1-AP, lot 1, rabbit polyclonal antibody, ProteinTech, dilution 1:300); anti-Crbn (catalog no. HPA045910, lot Q103829, rabbit polyclonal antibody, Sigma, dilution 1:500); and anti-FLAG M2 (catalog no. A2220, lot SLBF8148, mouse monoclonal antibody, Sigma, dilution 1:1000).

Techniques: Binding Assay

AL2CR, but not the AL1CR, binds CRL4CRBN via the substrate recognition pocket of Crbn. A, alignments of the ACR, AL1CR, and AL2CR show that the ACR and the AL2CR are the most conserved intracellular regions of the APP protein family. B, Western blotting analysis of pulldowns with brains isolated from WT mice shows that the AL2CR, but not the AL1CR, interacts with Cul4a, Ddb1, and Crbn. The WB shown is representative of two independent experiments. C, St-AL2CR binds Ddb1 in lysate from WT but not from Crbn-KO mice. The WB shown is representative of three independent experiments. D, Western blotting analysis of St-AL2CR pulldowns from brains isolated from WT mice shows that incubation of the lysates with either thalidomide and lenalidomide prior to pulldowns interferes with the AL2CR-Crbn interaction. The WB shown is representative of two independent experiments.

Journal: The Journal of Biological Chemistry

Article Title: Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4 CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration *

doi: 10.1074/jbc.M116.733626

Figure Lengend Snippet: AL2CR, but not the AL1CR, binds CRL4CRBN via the substrate recognition pocket of Crbn. A, alignments of the ACR, AL1CR, and AL2CR show that the ACR and the AL2CR are the most conserved intracellular regions of the APP protein family. B, Western blotting analysis of pulldowns with brains isolated from WT mice shows that the AL2CR, but not the AL1CR, interacts with Cul4a, Ddb1, and Crbn. The WB shown is representative of two independent experiments. C, St-AL2CR binds Ddb1 in lysate from WT but not from Crbn-KO mice. The WB shown is representative of three independent experiments. D, Western blotting analysis of St-AL2CR pulldowns from brains isolated from WT mice shows that incubation of the lysates with either thalidomide and lenalidomide prior to pulldowns interferes with the AL2CR-Crbn interaction. The WB shown is representative of two independent experiments.

Article Snippet: The following antibodies were used in WB: anti-Ddb1 (catalog no. 6998s, lot 1, rabbit monoclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Pin-1 (catalog no. 3722s, lot 3, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Stub1 (catalog no. 2080s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Grb2 (catalog no. 3972s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Cul4a (catalog no. 14851-1-AP, lot 1, rabbit polyclonal antibody, ProteinTech, dilution 1:300); anti-Crbn (catalog no. HPA045910, lot Q103829, rabbit polyclonal antibody, Sigma, dilution 1:500); and anti-FLAG M2 (catalog no. A2220, lot SLBF8148, mouse monoclonal antibody, Sigma, dilution 1:1000).

Techniques: Western Blot, Isolation, Incubation

UPS-related proteins, including Ddb1, Crbn, Stub1, Cul4a, and Cul4b, bind to the AL2CR but not to the AL1CR The table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); NSAF of pulldown of WT mouse brains with St alone (4th column); St-AL1CR (5th column); and NSAF of pulldown of WT mouse brains with St-AL2CR (6th column).

Journal: The Journal of Biological Chemistry

Article Title: Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4 CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration *

doi: 10.1074/jbc.M116.733626

Figure Lengend Snippet: UPS-related proteins, including Ddb1, Crbn, Stub1, Cul4a, and Cul4b, bind to the AL2CR but not to the AL1CR The table contains the list of proteins identified (1st column); the database accession numbers (2nd column); the molecular mass in kDa (3rd column); NSAF of pulldown of WT mouse brains with St alone (4th column); St-AL1CR (5th column); and NSAF of pulldown of WT mouse brains with St-AL2CR (6th column).

Article Snippet: The following antibodies were used in WB: anti-Ddb1 (catalog no. 6998s, lot 1, rabbit monoclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Pin-1 (catalog no. 3722s, lot 3, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Stub1 (catalog no. 2080s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Grb2 (catalog no. 3972s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Cul4a (catalog no. 14851-1-AP, lot 1, rabbit polyclonal antibody, ProteinTech, dilution 1:300); anti-Crbn (catalog no. HPA045910, lot Q103829, rabbit polyclonal antibody, Sigma, dilution 1:500); and anti-FLAG M2 (catalog no. A2220, lot SLBF8148, mouse monoclonal antibody, Sigma, dilution 1:1000).

Techniques:

In vitro ubiquitination of proteins present in the St-ACR Tyr(P)Thr(P) pulldowns 1st column lists some of the proteins ubiquitinated in vitro in an ACR Tyr(P)Thr(P) -dependent manner. 2nd column lists all the lysine residues found ubiquitinated in vitro. The K-ub found in our UbiScans from mouse brains are indicated with (m*) and in previous UbiScan experiments from mouse (m), human (h), rat (r) and chicken (c) tissues, which are reported online. 3rd column reports database accession numbers.

Journal: The Journal of Biological Chemistry

Article Title: Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4 CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration *

doi: 10.1074/jbc.M116.733626

Figure Lengend Snippet: In vitro ubiquitination of proteins present in the St-ACR Tyr(P)Thr(P) pulldowns 1st column lists some of the proteins ubiquitinated in vitro in an ACR Tyr(P)Thr(P) -dependent manner. 2nd column lists all the lysine residues found ubiquitinated in vitro. The K-ub found in our UbiScans from mouse brains are indicated with (m*) and in previous UbiScan experiments from mouse (m), human (h), rat (r) and chicken (c) tissues, which are reported online. 3rd column reports database accession numbers.

Article Snippet: The following antibodies were used in WB: anti-Ddb1 (catalog no. 6998s, lot 1, rabbit monoclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Pin-1 (catalog no. 3722s, lot 3, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Stub1 (catalog no. 2080s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:300); anti-Grb2 (catalog no. 3972s, lot 2, rabbit polyclonal antibody, Cell Signaling Technology, dilution 1:1000); anti-Cul4a (catalog no. 14851-1-AP, lot 1, rabbit polyclonal antibody, ProteinTech, dilution 1:300); anti-Crbn (catalog no. HPA045910, lot Q103829, rabbit polyclonal antibody, Sigma, dilution 1:500); and anti-FLAG M2 (catalog no. A2220, lot SLBF8148, mouse monoclonal antibody, Sigma, dilution 1:1000).

Techniques: In Vitro